Chromatography think B- This experiment is an abstract of the separation of show pigments. The analysis is make to examine the types of pigments found in spinach plant leaves mixed with acetone. The stems of the spinach were removed. The remaining leaves were squashed to discombobulateher in an acetone-filled howitzer employ a pestle. subsequently obtaining a putting green mush, chromatography was mend to be done. Three pieces of chromatography paper were do to a standardised form to the long pointed centrifuge tube-shaped structure. A capillary tube was cut at the end to absorb some of the green pigment. victimisation the tube, a dissipate of green was dabbed onto the different document 1 cm above the tip and dipped into a solvent-filled centrifuge tube. Each paper was dipped into one of trey solvents. The uncertain was controlled by making sure that each disperse was the fill same green so there was an lucifer optical view of the results. After 15-20 mins th e tube was checked. The distance from the raw(prenominal) dot to the original dot was cadenced in centimeters. The mixed new colors that appeared were taken note of and compared. Limiting Factors Planning A- The purpose of this experiment was to take down the presence of stiffen in two different types of leaves finished two types of methods. two limiting factors, chlorophyll and light, were observed.
Qualitative measuring was done to measure how much starch, if any at all, was produced and how the tack reacted to light. Planning B- twain plants, the coleus and plant #2, were starved of light and destarched for 72 hours. After being starved, a stencil! was applied to a foliation from plant #2 which was then attached to a lamp. solely parts of the leaf except the stencil were cover with aluminum foil to control the variable of light.
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